Detailed information of TA system TA128157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2511794..2512443
• Replicon: chromosome
• Accession: NZ_CP041354
• Organism: Pseudomonas aeruginosa strain AZPAE15042

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: IHMA87_RS12215
• Protein ID: WP_034082581.1
• Coordinates: 2512099..2512443 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IHMA87_RS12210
• Protein ID: WP_034082584.1
• Coordinates: 2511794..2512102 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IHMA87_RS32440	2507642..2507998	-	357	Protein_2349	hypothetical protein	-
IHMA87_RS12190	2508016..2508912	-	897	WP_013970892.1	LysR family transcriptional regulator	-
IHMA87_RS12195	2509053..2509937	+	885	Protein_2351	zinc-dependent alcohol dehydrogenase family protein	-
IHMA87_RS12200	2509975..2511201	-	1227	WP_010794468.1	IS256-like element ISPa1328 family transposase	-
IHMA87_RS12205	2511265..2511378	+	114	Protein_2353	zinc-binding dehydrogenase	-
IHMA87_RS12210	2511794..2512102	-	309	WP_034082584.1	transcriptional regulator	Antitoxin
IHMA87_RS12215	2512099..2512443	-	345	WP_034082581.1	toxin	Toxin
IHMA87_RS12220	2512602..2513528	-	927	WP_034082578.1	LysR family transcriptional regulator	-
IHMA87_RS12225	2513542..2514741	-	1200	WP_079383598.1	MFS transporter	-
IHMA87_RS12230	2514828..2516681	-	1854	WP_034082575.1	asparagine synthase (glutamine-hydrolyzing)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2466123..2559753	93630
	-	flank	IS/Tn	-	-	2509975..2511201	1226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-92)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13243.08 Da
• Isoelectric Point: 9.8528

>T128157 WP_034082581.1 NZ_CP041354:c2512443-2512099 [Pseudomonas aeruginosa]
MKALFVELSPFERNRKAYLDDGEYSLFQQMLLANPEAGAVIANSGGLRKVRFGSARRNKGKRGGVRVIYYYWHSGLQFWL
FTLYDKDELDDLSSDQRRQLKALLEHEVKARETP

Nucleotide

• Length: 345 bp

>T128157 NZ_CP041354:c2512443-2512099 [Pseudomonas aeruginosa]
ATGAAAGCATTGTTCGTTGAACTGTCACCCTTTGAGCGCAACCGAAAAGCCTACCTGGATGACGGCGAATACTCGCTATT
CCAACAGATGCTCTTGGCCAACCCGGAGGCAGGCGCAGTCATCGCCAACAGCGGTGGTCTGAGGAAGGTCCGCTTCGGAT
CGGCCAGGCGCAACAAAGGGAAGCGCGGTGGCGTTCGGGTGATTTATTACTACTGGCACAGCGGTCTGCAGTTCTGGCTC
TTCACCCTGTATGACAAAGACGAACTCGACGACCTGAGCAGTGACCAGCGTAGACAGCTGAAGGCGCTTCTTGAGCACGA
AGTGAAGGCGAGGGAAACACCATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.36 Da
• Isoelectric Point: 10.0927

>AT128157 WP_034082584.1 NZ_CP041354:c2512102-2511794 [Pseudomonas aeruginosa]
MTKRDIFSELIEGFDDLAAAREGKRTLRTHKVEYKPIEPATGAEIVALREKLQMSQGVFAQAIHAKPATLKNWEQNRSKP
NDQATVLIRILNKHPDLIRELA

Nucleotide

• Length: 309 bp

>AT128157 NZ_CP041354:c2512102-2511794 [Pseudomonas aeruginosa]
ATGACCAAACGCGATATTTTTTCCGAACTGATCGAAGGCTTCGACGACCTGGCCGCAGCCCGTGAAGGCAAGCGTACGCT
GCGTACCCACAAAGTCGAATACAAGCCAATCGAACCCGCGACAGGTGCGGAAATCGTCGCACTGCGCGAAAAGCTGCAGA
TGTCGCAGGGTGTCTTTGCCCAGGCCATTCACGCCAAGCCTGCAACCCTGAAGAACTGGGAACAGAACCGCTCAAAGCCC
AACGACCAGGCTACTGTTCTCATCCGTATCCTGAACAAGCATCCGGACCTCATACGCGAACTGGCGTAG