Detailed information of TA system TA128148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3880404..3881001
• Replicon: chromosome
• Accession: NZ_CP041353
• Organism: Klebsiella pneumoniae strain RJY9645

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: FJR44_RS18735
• Protein ID: WP_004893639.1
• Coordinates: 3880684..3881001 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: FJR44_RS18730
• Protein ID: WP_004142561.1
• Coordinates: 3880404..3880691 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJR44_RS18700	3876484..3876732	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
FJR44_RS18705	3876750..3877091	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
FJR44_RS18710	3877122..3878237	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
FJR44_RS18715	3878417..3878998	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
FJR44_RS18720	3878998..3879366	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
FJR44_RS18725	3879486..3880139	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
FJR44_RS18730	3880404..3880691	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
FJR44_RS18735	3880684..3881001	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FJR44_RS18740	3881186..3882229	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
FJR44_RS18745	3882895..3883761	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
FJR44_RS18750	3883870..3885297	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T128148 WP_004893639.1 NZ_CP041353:c3881001-3880684 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T128148 NZ_CP041353:c3881001-3880684 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAAATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT128148 WP_004142561.1 NZ_CP041353:c3880691-3880404 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT128148 NZ_CP041353:c3880691-3880404 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1