Detailed information of TA system TA128088

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26654..27080
• Replicon: plasmid pSUH-2
• Accession: NZ_CP041339
• Organism: Escherichia coli strain CCUG 73778

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FMA85_RS25740
• Protein ID: WP_001312861.1
• Coordinates: 26654..26812 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	26856..27080 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMA85_RS25710	21971..22792	-	822	WP_001234445.1	DUF945 domain-containing protein	-
FMA85_RS25715	22903..23199	-	297	WP_001272251.1	hypothetical protein	-
FMA85_RS26315	23591..23795	+	205	Protein_34	pilus protein	-
FMA85_RS25725	23880..25493	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
FMA85_RS25730	25524..25874	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
FMA85_RS25735	25871..26296	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
FMA85_RS25740	26654..26812	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	26856..27080	-	225	NuclAT_0	-	Antitoxin
-	26856..27080	-	225	NuclAT_0	-	Antitoxin
-	26856..27080	-	225	NuclAT_0	-	Antitoxin
-	26856..27080	-	225	NuclAT_0	-	Antitoxin
FMA85_RS25745	26892..27071	+	180	WP_001309233.1	hypothetical protein	-
FMA85_RS25750	27092..27811	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
FMA85_RS25755	27808..28242	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
FMA85_RS25760	28311..30334	-	2024	Protein_42	ParB/RepB/Spo0J family partition protein	-
FMA85_RS25765	30395..30628	-	234	WP_000006003.1	DUF905 family protein	-
FMA85_RS25770	30686..31213	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr	-	1..79945	79945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T128088 WP_001312861.1 NZ_CP041339:c26812-26654 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128088 NZ_CP041339:c26812-26654 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT128088 NZ_CP041339:c27080-26856 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin