Detailed information of TA system TA128084

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 139880..140301
• Replicon: plasmid pSUH-1
• Accession: NZ_CP041338
• Organism: Escherichia coli strain CCUG 73778

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: FMA85_RS25415
• Protein ID: WP_001302184.1
• Coordinates: 139880..140038 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	140103..140301 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMA85_RS25380	134944..135159	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
FMA85_RS25385	135295..135942	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
FMA85_RS25390	136133..136516	-	384	WP_001063020.1	relaxosome protein TraM	-
FMA85_RS25395	136886..137458	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
FMA85_RS25400	137754..138575	-	822	WP_001234465.1	DUF945 domain-containing protein	-
FMA85_RS25405	138694..138981	-	288	WP_000107242.1	hypothetical protein	-
FMA85_RS26310	139006..139212	-	207	WP_000275854.1	hypothetical protein	-
FMA85_RS25415	139880..140038	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	140103..140301	-	199	NuclAT_0	-	Antitoxin
-	140103..140301	-	199	NuclAT_0	-	Antitoxin
-	140103..140301	-	199	NuclAT_0	-	Antitoxin
-	140103..140301	-	199	NuclAT_0	-	Antitoxin
FMA85_RS25420	140270..140375	-	106	Protein_161	plasmid SOS inhibition protein A	-
FMA85_RS25425	140530..140847	+	318	Protein_162	IS110 family transposase	-
FMA85_RS25430	141136..141387	-	252	Protein_163	iron-containing alcohol dehydrogenase	-
FMA85_RS25435	141472..142095	-	624	WP_000912556.1	potassium channel family protein	-
FMA85_RS25440	142111..142804	-	694	Protein_165	IS1 family transposase	-
FMA85_RS25445	142861..142965	+	105	Protein_166	thiosulfate reductase cytochrome B subunit	-
FMA85_RS25450	143211..144605	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA5 / blaTEM-1B	-	1..161372	161372
	-	flank	IS/Tn	-	-	142111..142488	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T128084 WP_001302184.1 NZ_CP041338:c140038-139880 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T128084 NZ_CP041338:c140038-139880 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT128084 NZ_CP041338:c140301-140103 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin