Detailed information of TA system TA128082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102463..102717
• Replicon: plasmid pSUH-1
• Accession: NZ_CP041338
• Organism: Escherichia coli strain CCUG 73778

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FMA85_RS25195
• Protein ID: WP_015387399.1
• Coordinates: 102463..102669 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	102656..102717 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMA85_RS25165	97710..98621	-	912	WP_000440183.1	carbamate kinase	-
FMA85_RS25170	98632..99852	-	1221	WP_000410951.1	arginine deiminase	-
FMA85_RS25175	100745..101227	+	483	Protein_112	VENN motif pre-toxin domain-containing protein	-
FMA85_RS25180	101173..101619	-	447	Protein_113	incFII family plasmid replication initiator RepA	-
FMA85_RS25185	101612..101686	-	75	WP_001365705.1	RepA leader peptide Tap	-
FMA85_RS25190	101922..102179	-	258	WP_000083833.1	replication regulatory protein RepA	-
FMA85_RS25195	102463..102669	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	102656..102717	+	62	NuclAT_1	-	Antitoxin
-	102656..102717	+	62	NuclAT_1	-	Antitoxin
-	102656..102717	+	62	NuclAT_1	-	Antitoxin
-	102656..102717	+	62	NuclAT_1	-	Antitoxin
FMA85_RS25200	102856..103038	-	183	WP_000968309.1	hypothetical protein	-
FMA85_RS25205	103139..103841	+	703	Protein_118	IS1 family transposase	-
FMA85_RS25210	104142..104354	-	213	WP_005012601.1	hypothetical protein	-
FMA85_RS25215	104487..105047	-	561	WP_000139363.1	fertility inhibition protein FinO	-
FMA85_RS25220	105102..105848	-	747	WP_000205701.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / sul1 / qacE / aadA5 / blaTEM-1B	-	1..161372	161372
	-	flank	IS/Tn	-	-	103455..103841	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T128082 WP_015387399.1 NZ_CP041338:c102669-102463 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T128082 NZ_CP041338:c102669-102463 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT128082 NZ_CP041338:102656-102717 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin