Detailed information of TA system TA128072

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3593217..3593819
• Replicon: chromosome
• Accession: NZ_CP041337
• Organism: Escherichia coli strain CCUG 73778

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: FMA85_RS17515
• Protein ID: WP_000897302.1
• Coordinates: 3593508..3593819 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FMA85_RS17510
• Protein ID: WP_000356397.1
• Coordinates: 3593217..3593507 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FMA85_RS17480	3588524..3589453	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
FMA85_RS17485	3589635..3589877	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
FMA85_RS17490	3590167..3591015	+	849	WP_001038650.1	hypothetical protein	-
FMA85_RS17495	3591331..3591780	+	450	WP_032140890.1	hypothetical protein	-
FMA85_RS17500	3591965..3592183	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
FMA85_RS17505	3592580..3592858	-	279	WP_001296612.1	hypothetical protein	-
FMA85_RS17510	3593217..3593507	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FMA85_RS17515	3593508..3593819	-	312	WP_000897302.1	hypothetical protein	Toxin
FMA85_RS17520	3594048..3594956	+	909	WP_001385591.1	alpha/beta hydrolase	-
FMA85_RS17525	3595020..3595961	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FMA85_RS17530	3596006..3596443	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FMA85_RS17535	3596440..3597312	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FMA85_RS17540	3597306..3597905	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T128072 WP_000897302.1 NZ_CP041337:c3593819-3593508 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128072 NZ_CP041337:c3593819-3593508 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128072 WP_000356397.1 NZ_CP041337:c3593507-3593217 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128072 NZ_CP041337:c3593507-3593217 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416