Detailed information of TA system TA128049

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1645937..1646580
• Replicon: chromosome
• Accession: NZ_CP041334
• Organism: Glaesserella parasuis strain HPS412

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: FLK62_RS08070
• Protein ID: WP_005714197.1
• Coordinates: 1646236..1646580 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FLK62_RS08065
• Protein ID: WP_012621770.1
• Coordinates: 1645937..1646233 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLK62_RS08040	1641267..1641515	-	249	WP_160429493.1	hypothetical protein	-
FLK62_RS08045	1641478..1643406	-	1929	WP_176443754.1	ATP-dependent zinc metalloprotease FtsH	-
FLK62_RS08050	1643480..1644106	-	627	WP_010786866.1	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	-
FLK62_RS08055	1644349..1645137	+	789	WP_005714049.1	SDR family oxidoreductase	-
FLK62_RS08060	1645217..1645879	+	663	WP_010786865.1	Fic family protein	-
FLK62_RS08065	1645937..1646233	-	297	WP_012621770.1	helix-turn-helix domain-containing protein	Antitoxin
FLK62_RS08070	1646236..1646580	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FLK62_RS08075	1646795..1648774	+	1980	WP_176443755.1	exoribonuclease II	-
FLK62_RS08080	1648885..1649976	+	1092	WP_005714201.1	murein transglycosylase A	-
FLK62_RS08085	1650442..1651044	+	603	WP_176444014.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T128049 WP_005714197.1 NZ_CP041334:c1646580-1646236 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T128049 NZ_CP041334:c1646580-1646236 [Glaesserella parasuis]
ATGGAAACAGAAGAATATCTTACTTTTATTGAAACCAAAGTTTTTGAAGAAGACCGAAAAGCATTAATGTCTGATGATGA
ATATCAGAAATTTCAGGCTTATTTATTAGAATCTCACGAATTAGGCGATTTTATTCAGAATACTGGTGGTTGCCAAAAAA
TTCGCTGGAAACTAGAAAGTAATAACAAGGGCAAAAGTGGTGGTGTAAGGGTGATTTACTATGTTGTAACCAAACAGGGA
AAATTATTATTAATGATGATGTATGCTAAAAGCAAACAAGACAATATGAGTGATAAACAAAAAGCAATGTTAAAAGCGGT
TGTATCTCAATTAAGTGAGGAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11224.13 Da
• Isoelectric Point: 10.0887

>AT128049 WP_012621770.1 NZ_CP041334:c1646233-1645937 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPNLVPVLQNL

Nucleotide

• Length: 297 bp

>AT128049 NZ_CP041334:c1646233-1645937 [Glaesserella parasuis]
ATGGAAAAAGAACTTTTTGAACGTCTAATGGAAAGTGCGAGAGAAATGGTCGCCATTGAAAAAGGCGAAATAGAAGTACC
ACCGCATCGTATTCATATCCATAAAATCCCGAATGTCAAAGCGATTAGAACCCAGCACCAGATGAAACAAGCGGAATTTG
CTTCCCTTTTAGGCGTAAGCCAAAATCTTGTGCAGTCGTGGGAAATTGGCAGACGAACCCCAAATGGCGCTGCACGGAAA
TTGCTTGCGATTATTGAGCAACAGCCTAATCTTGTCCCTGTACTTCAGAATTTATAA

Structures

Toxin

• AlphaFold DB: U4SMB1