Detailed information of TA system TA128042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4725651..4726253
• Replicon: chromosome
• Accession: NZ_CP041304
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FK544_RS24410
• Protein ID: WP_000897305.1
• Coordinates: 4725942..4726253 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FK544_RS24405
• Protein ID: WP_000356397.1
• Coordinates: 4725651..4725941 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS24385	4722153..4723055	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FK544_RS24390	4723052..4723687	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FK544_RS24395	4723684..4724613	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FK544_RS24400	4724828..4725046	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FK544_RS24405	4725651..4725941	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FK544_RS24410	4725942..4726253	-	312	WP_000897305.1	hypothetical protein	Toxin
FK544_RS24415	4726482..4727390	+	909	WP_001318161.1	alpha/beta hydrolase	-
FK544_RS24420	4727454..4728395	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FK544_RS24425	4728440..4728877	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FK544_RS24430	4728874..4729746	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FK544_RS24435	4729740..4730339	-	600	WP_001315111.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128042 WP_000897305.1 NZ_CP041304:c4726253-4725942 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128042 NZ_CP041304:c4726253-4725942 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128042 WP_000356397.1 NZ_CP041304:c4725941-4725651 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128042 NZ_CP041304:c4725941-4725651 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7