Detailed information of TA system TA128010

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122431..122685
• Replicon: plasmid pCys-11
• Accession: NZ_CP041303
• Organism: Escherichia coli strain MSHS 133

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FK544_RS00800
• Protein ID: WP_001351576.1
• Coordinates: 122431..122637 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	122624..122685 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK544_RS00755	118186..118590	-	405	WP_000154256.1	DUF2251 domain-containing protein	-
FK544_RS00760	118760..119047	-	288	WP_000222774.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FK544_RS00765	119044..119295	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FK544_RS25535	119419..119559	-	141	WP_161953058.1	hypothetical protein	-
FK544_RS00775	120259..121116	-	858	WP_000774296.1	incFII family plasmid replication initiator RepA	-
FK544_RS00780	121109..121591	-	483	WP_001273588.1	hypothetical protein	-
FK544_RS00785	121584..121658	-	75	WP_001442103.1	RepA leader peptide Tap	-
FK544_RS00795	121890..122147	-	258	WP_000083845.1	replication regulatory protein RepA	-
FK544_RS00800	122431..122637	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	122624..122685	+	62	NuclAT_1	-	Antitoxin
-	122624..122685	+	62	NuclAT_1	-	Antitoxin
-	122624..122685	+	62	NuclAT_1	-	Antitoxin
-	122624..122685	+	62	NuclAT_1	-	Antitoxin
FK544_RS25630	122941..123015	-	75	Protein_139	endonuclease	-
FK544_RS00810	123261..123473	-	213	WP_001299730.1	ANR family transcriptional regulator	-
FK544_RS00815	123609..124169	-	561	WP_000139315.1	fertility inhibition protein FinO	-
FK544_RS00820	124272..125132	-	861	WP_000704513.1	alpha/beta hydrolase	-
FK544_RS00825	125191..125937	-	747	WP_000205741.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroD / iroE / iroN	1..157660	157660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T128010 WP_001351576.1 NZ_CP041303:c122637-122431 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T128010 NZ_CP041303:c122637-122431 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT128010 NZ_CP041303:122624-122685 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin