Detailed information of TA system TA128000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4584690..4585292
• Replicon: chromosome
• Accession: NZ_CP041302
• Organism: Escherichia coli strain MSHS 472

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FK543_RS23285
• Protein ID: WP_000897305.1
• Coordinates: 4584981..4585292 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FK543_RS23280
• Protein ID: WP_000356397.1
• Coordinates: 4584690..4584980 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK543_RS23255	4580635..4581537	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
FK543_RS23260	4581534..4582169	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FK543_RS23265	4582166..4583095	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FK543_RS23270	4583425..4583667	-	243	WP_001087409.1	hypothetical protein	-
FK543_RS23275	4583886..4584104	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
FK543_RS23280	4584690..4584980	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FK543_RS23285	4584981..4585292	-	312	WP_000897305.1	hypothetical protein	Toxin
FK543_RS23290	4585521..4586429	+	909	WP_001162704.1	alpha/beta hydrolase	-
FK543_RS23295	4586493..4587434	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FK543_RS23300	4587479..4587916	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FK543_RS23305	4587913..4588785	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FK543_RS23310	4588779..4589378	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T128000 WP_000897305.1 NZ_CP041302:c4585292-4584981 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T128000 NZ_CP041302:c4585292-4584981 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT128000 WP_000356397.1 NZ_CP041302:c4584980-4584690 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT128000 NZ_CP041302:c4584980-4584690 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7