Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3735831..3736372 Replicon chromosome
Accession NZ_CP041302
Organism Escherichia coli strain MSHS 472

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag FK543_RS19265 Protein ID WP_000615982.1
Coordinates 3736094..3736372 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FK543_RS19260 Protein ID WP_000729704.1
Coordinates 3735831..3736091 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK543_RS19235 3730867..3732006 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
FK543_RS19240 3731990..3734083 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
FK543_RS19245 3734188..3734466 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FK543_RS19250 3734459..3734815 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FK543_RS19255 3734872..3735645 - 774 WP_000543899.1 C40 family peptidase -
FK543_RS19260 3735831..3736091 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FK543_RS19265 3736094..3736372 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FK543_RS19270 3736528..3737268 + 741 WP_001225679.1 murein L,D-transpeptidase -
FK543_RS19275 3737239..3738006 - 768 WP_000333380.1 class II glutamine amidotransferase -
FK543_RS19280 3738212..3738790 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T127995 WP_000615982.1 NZ_CP041302:3736094-3736372 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T127995 NZ_CP041302:3736094-3736372 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT127995 WP_000729704.1 NZ_CP041302:3735831-3736091 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT127995 NZ_CP041302:3735831-3736091 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References