Detailed information of TA system TA127973

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8779..9205
• Replicon: plasmid pCys-6
• Accession: NZ_CP041301
• Organism: Escherichia coli strain MSHS 472

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FK543_RS00080
• Protein ID: WP_001312861.1
• Coordinates: 8779..8937 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	8981..9205 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK543_RS00040	4151..4840	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
FK543_RS00045	5027..5410	-	384	WP_053320747.1	relaxosome protein TraM	-
FK543_RS00050	5730..6332	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
FK543_RS00055	6629..7450	-	822	WP_001234469.1	DUF945 domain-containing protein	-
FK543_RS00060	7570..7857	-	288	WP_000107535.1	hypothetical protein	-
FK543_RS24375	7882..8088	-	207	WP_000547939.1	hypothetical protein	-
FK543_RS00080	8779..8937	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	8981..9205	-	225	NuclAT_0	-	Antitoxin
-	8981..9205	-	225	NuclAT_0	-	Antitoxin
-	8981..9205	-	225	NuclAT_0	-	Antitoxin
-	8981..9205	-	225	NuclAT_0	-	Antitoxin
FK543_RS24250	9017..9205	+	189	WP_001299721.1	hypothetical protein	-
FK543_RS00085	9217..9936	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
FK543_RS00090	9933..10367	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
FK543_RS00095	10422..12380	-	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
FK543_RS00100	12446..12679	-	234	WP_000005990.1	DUF905 family protein	-
FK543_RS00105	12742..13281	-	540	WP_000290791.1	single-stranded DNA-binding protein	-
FK543_RS24255	13479..13739	-	261	WP_071600428.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / dfrA12 / aadA2 / qacE / sul1 / mph(A) / tet(A)	iroB / iroC / iroD / iroE / iroN	1..150137	150137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T127973 WP_001312861.1 NZ_CP041301:c8937-8779 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T127973 NZ_CP041301:c8937-8779 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT127973 NZ_CP041301:c9205-8981 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin