Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3958155..3958849 Replicon chromosome
Accession NZ_CP041300
Organism Escherichia coli O1:H42 strain CLSC36

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag FK541_RS20285 Protein ID WP_001263491.1
Coordinates 3958155..3958553 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag FK541_RS20290 Protein ID WP_000554755.1
Coordinates 3958556..3958849 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FK541_RS20255 3953417..3954874 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
FK541_RS20260 3954883..3955164 + 282 WP_022645226.1 hypothetical protein -
FK541_RS20265 3955181..3955690 - 510 WP_001361775.1 hydrolase -
FK541_RS20270 3955752..3956366 - 615 WP_022645225.1 peptide chain release factor H -
FK541_RS20275 3956363..3957502 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
FK541_RS20280 3957693..3958145 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
FK541_RS20285 3958155..3958553 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
FK541_RS20290 3958556..3958849 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
FK541_RS20295 3958901..3959956 - 1056 WP_022645222.1 DNA polymerase IV -
FK541_RS20300 3960027..3960812 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
FK541_RS20305 3960784..3962498 + 1715 Protein_3714 flagellar biosynthesis protein FlhA -
FK541_RS20310 3962596..3963369 - 774 WP_022645219.1 C40 family peptidase -
FK541_RS20315 3963555..3963815 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T127968 WP_001263491.1 NZ_CP041300:c3958553-3958155 [Escherichia coli O1:H42]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T127968 NZ_CP041300:c3958553-3958155 [Escherichia coli O1:H42]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT127968 WP_000554755.1 NZ_CP041300:c3958849-3958556 [Escherichia coli O1:H42]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT127968 NZ_CP041300:c3958849-3958556 [Escherichia coli O1:H42]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References