Detailed information of TA system TA127938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 29332..29983
• Replicon: plasmid p80-1-2-p2
• Accession: NZ_CP041296
• Organism: Acinetobacter indicus strain 80-1-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: FK538_RS14185
• Protein ID: WP_004668307.1
• Coordinates: 29332..29688 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FK538_RS14190
• Protein ID: WP_005237493.1
• Coordinates: 29681..29983 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK538_RS14170	25592..27457	+	1866	WP_104470970.1	copper resistance system multicopper oxidase	-
FK538_RS14175	27444..27782	+	339	WP_004897649.1	hypothetical protein	-
FK538_RS14180	27769..28713	+	945	WP_104510421.1	copper resistance protein B	-
FK538_RS14185	29332..29688	+	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FK538_RS14190	29681..29983	+	303	WP_005237493.1	helix-turn-helix transcriptional regulator	Antitoxin
FK538_RS14195	30025..30186	-	162	Protein_24	IS6 family transposase	-
FK538_RS14200	30280..30672	-	393	WP_000550240.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
FK538_RS14205	30762..32042	+	1281	WP_005237495.1	cation transporter	-
FK538_RS14210	32106..32810	+	705	WP_168370865.1	IS6-like element IS1006 family transposase	-
FK538_RS14215	32878..33852	-	975	WP_160243457.1	calcium/sodium antiporter	-
FK538_RS14220	33960..34120	+	161	Protein_29	DUF475 domain-containing protein	-
FK538_RS14225	34114..34638	+	525	WP_160243456.1	TIGR00645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39796	39796

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T127938 WP_004668307.1 NZ_CP041296:29332-29688 [Acinetobacter indicus]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T127938 NZ_CP041296:29332-29688 [Acinetobacter indicus]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11208.99 Da
• Isoelectric Point: 6.4759

>AT127938 WP_005237493.1 NZ_CP041296:29681-29983 [Acinetobacter indicus]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTLKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT127938 NZ_CP041296:29681-29983 [Acinetobacter indicus]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCTTGAAAAAGTATGTTGAGGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: N9NM16