Detailed information of TA system TA127929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3663712..3664406
• Replicon: chromosome
• Accession: NZ_CP041284
• Organism: Escherichia coli strain 54

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FK536_RS17760
• Protein ID: WP_001263493.1
• Coordinates: 3663712..3664110 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FK536_RS17765
• Protein ID: WP_000554757.1
• Coordinates: 3664113..3664406 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FK536_RS17730	3659271..3659729	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FK536_RS17735	3659990..3661447	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FK536_RS17740	3661504..3662025	-	522	Protein_3467	peptide chain release factor H	-
FK536_RS17745	3662024..3662227	-	204	Protein_3468	RNA ligase RtcB family protein	-
FK536_RS17750	3662473..3662925	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FK536_RS17755	3662999..3663696	+	698	Protein_3470	IS1 family transposase	-
FK536_RS17760	3663712..3664110	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FK536_RS17765	3664113..3664406	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FK536_RS17770	3664458..3665513	-	1056	WP_001226164.1	DNA polymerase IV	-
FK536_RS17775	3665584..3666369	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
FK536_RS17780	3666341..3668053	+	1713	Protein_3475	flagellar biosynthesis protein FlhA	-
FK536_RS17785	3668277..3668774	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3663403..3663696	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T127929 WP_001263493.1 NZ_CP041284:c3664110-3663712 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T127929 NZ_CP041284:c3664110-3663712 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT127929 WP_000554757.1 NZ_CP041284:c3664406-3664113 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT127929 NZ_CP041284:c3664406-3664113 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6