Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 180780..181315 Replicon plasmid pVVEswe-S1
Accession NZ_CP041271
Organism Enterococcus faecium strain VVEswe-S

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag FLC01_RS14805 Protein ID WP_002314366.1
Coordinates 180780..181052 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag FLC01_RS14810 Protein ID WP_002297422.1
Coordinates 181052..181315 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLC01_RS14770 176336..176979 + 644 Protein_176 transposase -
FLC01_RS14775 177055..177735 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
FLC01_RS14780 177790..178146 - 357 Protein_178 hypothetical protein -
FLC01_RS14785 178406..178954 - 549 WP_002292681.1 hypothetical protein -
FLC01_RS14790 178955..179812 - 858 WP_002292680.1 AAA family ATPase -
FLC01_RS14795 180098..180384 - 287 Protein_181 type II toxin-antitoxin system RelE/ParE family toxin -
FLC01_RS14800 180374..180703 - 330 WP_002292678.1 AbrB family transcriptional regulator -
FLC01_RS14805 180780..181052 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
FLC01_RS14810 181052..181315 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FLC01_RS14815 181549..182457 + 909 Protein_185 IS982-like element ISEfm1 family transposase -
FLC01_RS14820 182460..183635 - 1176 WP_060811363.1 MFS transporter -
FLC01_RS14825 183638..185887 - 2250 WP_060811364.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..194168 194168
- inside IScluster/Tn - - 168602..191916 23314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T127908 WP_002314366.1 NZ_CP041271:c181052-180780 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T127908 NZ_CP041271:c181052-180780 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT127908 WP_002297422.1 NZ_CP041271:c181315-181052 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT127908 NZ_CP041271:c181315-181052 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References