127902

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 182017..182552 Replicon plasmid pVVEswe-R1
Accession NZ_CP041262
Organism Enterococcus faecium strain VVEswe-R

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag FKW92_RS14800 Protein ID WP_002314366.1
Coordinates 182017..182289 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag FKW92_RS14805 Protein ID WP_002297422.1
Coordinates 182289..182552 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKW92_RS14765 177573..178216 + 644 Protein_176 transposase -
FKW92_RS14770 178292..178972 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
FKW92_RS14775 179027..179383 - 357 Protein_178 hypothetical protein -
FKW92_RS14780 179643..180191 - 549 WP_002292681.1 hypothetical protein -
FKW92_RS14785 180192..181049 - 858 WP_002292680.1 AAA family ATPase -
FKW92_RS14790 181335..181621 - 287 Protein_181 type II toxin-antitoxin system RelE/ParE family toxin -
FKW92_RS14795 181611..181940 - 330 WP_002292678.1 AbrB family transcriptional regulator -
FKW92_RS14800 182017..182289 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
FKW92_RS14805 182289..182552 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FKW92_RS14810 182786..183694 + 909 WP_002342382.1 IS982-like element ISEfm1 family transposase -
FKW92_RS14815 183697..184872 - 1176 WP_060811363.1 MFS transporter -
FKW92_RS14820 184875..187124 - 2250 WP_060811364.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..195404 195404
- inside IScluster/Tn - - 168593..193153 24560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T127902 WP_002314366.1 NZ_CP041262:c182289-182017 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T127902 NZ_CP041262:c182289-182017 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT127902 WP_002297422.1 NZ_CP041262:c182552-182289 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT127902 NZ_CP041262:c182552-182289 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References