Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 13313..13848 Replicon plasmid p26
Accession NZ_CP041256
Organism Enterococcus faecium strain 515

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag FKZ20_RS00785 Protein ID WP_002305108.1
Coordinates 13576..13848 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag FKZ20_RS00780 Protein ID WP_002303154.1
Coordinates 13313..13576 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FKZ20_RS00755 9316..9588 + 273 WP_002305107.1 DUF3884 family protein -
FKZ20_RS00760 9609..10508 + 900 WP_002303235.1 aldose 1-epimerase family protein -
FKZ20_RS00770 12222..12785 + 564 Protein_13 IS6 family transposase -
FKZ20_RS00775 12791..13207 + 417 WP_002303153.1 hypothetical protein -
FKZ20_RS00780 13313..13576 + 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
FKZ20_RS00785 13576..13848 + 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
FKZ20_RS00790 13925..14254 + 330 WP_002292678.1 AbrB family transcriptional regulator -
FKZ20_RS00795 14244..14531 + 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
FKZ20_RS00800 14817..15674 + 858 WP_002292680.1 AAA family ATPase -
FKZ20_RS00805 15675..16223 + 549 WP_002292681.1 hypothetical protein -
FKZ20_RS00810 16520..17467 - 948 WP_002303155.1 DNA-binding transcriptional regulator -
FKZ20_RS00815 17655..18842 + 1188 WP_002303156.1 NADP-dependent malic enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..150610 150610


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T127894 WP_002305108.1 NZ_CP041256:13576-13848 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T127894 NZ_CP041256:13576-13848 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT127894 WP_002303154.1 NZ_CP041256:13313-13576 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT127894 NZ_CP041256:13313-13576 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References