Detailed information of TA system TA127737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 59170..59632
• Replicon: plasmid pCUVET596
• Accession: NZ_CP041149
• Organism: Acinetobacter baumannii strain CUVET-MIC596

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: FI878_RS19795
• Protein ID: WP_000033474.1
• Coordinates: 59170..59358 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FI878_RS19800
• Protein ID: WP_114189641.1
• Coordinates: 59345..59632 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FI878_RS19760	55577..55933	+	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FI878_RS19765	55926..56228	+	303	WP_001140619.1	XRE family transcriptional regulator	-
FI878_RS19770	56221..56430	+	210	WP_000069471.1	hypothetical protein	-
FI878_RS19775	56540..56767	-	228	WP_005071544.1	hypothetical protein	-
FI878_RS19780	57526..58071	-	546	Protein_47	IS5 family transposase	-
FI878_RS19785	57999..58387	-	389	Protein_48	AAA family ATPase	-
FI878_RS19790	58573..58893	+	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FI878_RS19795	59170..59358	+	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FI878_RS19800	59345..59632	+	288	WP_114189641.1	helix-turn-helix domain-containing protein	Antitoxin
FI878_RS19805	59916..60140	-	225	WP_016653391.1	hypothetical protein	-
FI878_RS19810	60161..60592	-	432	WP_002033982.1	hypothetical protein	-
FI878_RS19815	60761..62077	-	1317	WP_114189640.1	terminase	-
FI878_RS19820	62061..62602	-	542	Protein_55	DUF2280 domain-containing protein	-
FI878_RS19825	62662..63303	-	642	WP_114189639.1	hypothetical protein	-
FI878_RS19830	63272..63739	-	468	WP_005071563.1	hypothetical protein	-
FI878_RS19835	63800..64255	-	456	WP_005071565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..82016	82016
	-	flank	IS/Tn	-	-	57526..57771	245
	-	flank	IS/Tn	-	-	64593..65525	932

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T127737 WP_000033474.1 NZ_CP041149:59170-59358 [Acinetobacter baumannii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T127737 NZ_CP041149:59170-59358 [Acinetobacter baumannii]
ATGAGTAAAGGACTATTTGAGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAA
ACGCATTGTAATGCTCCATAGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCA
TGAAAGAGGTGAAAAATGCGAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10593.35 Da
• Isoelectric Point: 9.7308

>AT127737 WP_114189641.1 NZ_CP041149:59345-59632 [Acinetobacter baumannii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSITTLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT127737 NZ_CP041149:59345-59632 [Acinetobacter baumannii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCTTGCTCGATATGGTACTTACTGCACTTCGAGAGGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAATGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTACCACATTGCAA
AAATACGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35