Detailed information of TA system TA127634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 533569..534222
• Replicon: chromosome
• Accession: NZ_CP041123
• Organism: Serratia marcescens strain WVU-002

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FBF86_RS02445
• Protein ID: WP_141957860.1
• Coordinates: 533569..533919 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: FBF86_RS02450
• Protein ID: WP_019453270.1
• Coordinates: 533923..534222 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FBF86_RS02415	529123..529533	-	411	WP_060440842.1	GNAT family N-acetyltransferase	-
FBF86_RS02420	529563..530933	-	1371	WP_060440841.1	glutamine synthetase family protein	-
FBF86_RS02425	531179..531496	+	318	WP_041037744.1	hypothetical protein	-
FBF86_RS02430	531487..531882	-	396	WP_064291098.1	hypothetical protein	-
FBF86_RS02435	531896..532288	-	393	WP_141957856.1	hypothetical protein	-
FBF86_RS02440	532292..533296	-	1005	WP_141957858.1	hypothetical protein	-
FBF86_RS02445	533569..533919	+	351	WP_141957860.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FBF86_RS02450	533923..534222	+	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
FBF86_RS02455	534212..535129	-	918	WP_141957862.1	LysR family transcriptional regulator	-
FBF86_RS02460	535298..535996	+	699	WP_049212496.1	CoA transferase subunit A	-
FBF86_RS02465	536008..536661	+	654	WP_019453268.1	CoA transferase subunit B	-
FBF86_RS02470	536676..537860	+	1185	WP_141957864.1	acetyl-CoA C-acetyltransferase	-
FBF86_RS02475	537879..538802	+	924	WP_060422138.1	3-hydroxyacyl-CoA dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13546.81 Da
• Isoelectric Point: 8.2699

>T127634 WP_141957860.1 NZ_CP041123:533569-533919 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLKGPLHSNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T127634 NZ_CP041123:533569-533919 [Serratia marcescens]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAACAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCAGGGCCTTCTCTTCGGCGGCCTTTTGTCGACGTGCTGAAAGGCCCGTTGCATTCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTTGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGCCCATCGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT127634 WP_019453270.1 NZ_CP041123:533923-534222 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT127634 NZ_CP041123:533923-534222 [Serratia marcescens]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAATTGGCGAAAACGCTGGGCATTTCCC
AGCCTTCTGTCGCGGCCATCGAAGGGCGCGGCAATGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3