Detailed information of TA system TA127624

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65044..65470
• Replicon: plasmid unnamed1
• Accession: NZ_CP041111
• Organism: Escherichia coli strain ECCTRSRTH03

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CI675_RS22975
• Protein ID: WP_001312861.1
• Coordinates: 65044..65202 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65246..65470 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI675_RS22935	60414..61103	-	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-
CI675_RS22940	61290..61673	-	384	WP_001151524.1	relaxosome protein TraM	-
CI675_RS22945	61993..62595	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
CI675_RS22950	62892..63713	-	822	WP_072795862.1	DUF945 domain-containing protein	-
CI675_RS22955	63835..64122	-	288	WP_000107535.1	hypothetical protein	-
CI675_RS22975	65044..65202	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65246..65470	-	225	NuclAT_0	-	Antitoxin
-	65246..65470	-	225	NuclAT_0	-	Antitoxin
-	65246..65470	-	225	NuclAT_0	-	Antitoxin
-	65246..65470	-	225	NuclAT_0	-	Antitoxin
CI675_RS24940	65282..65470	+	189	WP_001299721.1	hypothetical protein	-
CI675_RS22980	65482..66201	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
CI675_RS22985	66198..66632	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CI675_RS22990	66687..68645	-	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
CI675_RS22995	68710..68943	-	234	WP_000005987.1	DUF905 family protein	-
CI675_RS23000	69000..69539	-	540	WP_032332913.1	single-stranded DNA-binding protein	-
CI675_RS23005	69565..69771	-	207	WP_000275853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA2 / blaCTX-M-55 / mph(A) / erm(B) / aac(3)-IId	-	1..96497	96497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T127624 WP_001312861.1 NZ_CP041111:c65202-65044 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T127624 NZ_CP041111:c65202-65044 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT127624 NZ_CP041111:c65470-65246 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin