Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1782024..1782526 Replicon chromosome
Accession NZ_CP041110
Organism Escherichia coli strain ECCTRSRTH03

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CI675_RS08440 Protein ID WP_000767829.1
Coordinates 1782272..1782526 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CI675_RS08435 Protein ID WP_001259255.1
Coordinates 1782024..1782275 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CI675_RS08410 1777202..1778269 - 1068 WP_000080091.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CI675_RS08415 1778269..1779339 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
CI675_RS08420 1779336..1780640 - 1305 WP_001565639.1 histidinol dehydrogenase -
CI675_RS08425 1780646..1781545 - 900 WP_000131777.1 ATP phosphoribosyltransferase -
CI675_RS08430 1781691..1781741 - 51 WP_001364200.1 his operon leader peptide -
CI675_RS08435 1782024..1782275 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CI675_RS08440 1782272..1782526 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CI675_RS08445 1782609..1783433 + 825 WP_000754749.1 SDR family oxidoreductase -
CI675_RS08450 1783479..1784408 + 930 WP_000803351.1 LysR family transcriptional regulator -
CI675_RS08455 1784623..1784685 + 63 WP_010723108.1 membrane protein YoeI -
CI675_RS08460 1784675..1786033 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CI675_RS08465 1786212..1787270 + 1059 WP_000492321.1 transport protein YeeE -
CI675_RS08470 1787284..1787511 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T127604 WP_000767829.1 NZ_CP041110:1782272-1782526 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T127604 NZ_CP041110:1782272-1782526 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT127604 WP_001259255.1 NZ_CP041110:1782024-1782275 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT127604 NZ_CP041110:1782024-1782275 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References