Detailed information of TA system TA127595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 27800..28452
• Replicon: plasmid unnamed5
• Accession: NZ_CP041104
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPCTRSRTH07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B2CBE9
• Locus tag: CI661_RS29010
• Protein ID: WP_012457127.1
• Coordinates: 27800..28150 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: B2CBE8
• Locus tag: CI661_RS29015
• Protein ID: WP_012457126.1
• Coordinates: 28153..28452 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CI661_RS28985	23470..23784	-	315	Protein_29	DUF4158 domain-containing protein	-
CI661_RS28990	23787..24347	-	561	WP_094309425.1	recombinase family protein	-
CI661_RS28995	24710..26332	+	1623	WP_065813436.1	ATP-binding cassette domain-containing protein	-
CI661_RS29000	26495..26734	-	240	WP_012457129.1	ParG	-
CI661_RS29005	26789..27406	-	618	WP_094309423.1	ParA family protein	-
CI661_RS29010	27800..28150	+	351	WP_012457127.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CI661_RS29015	28153..28452	+	300	WP_012457126.1	helix-turn-helix domain-containing protein	Antitoxin
CI661_RS29920	28514..28663	-	150	WP_162262154.1	hypothetical protein	-
CI661_RS29020	28826..29491	-	666	WP_094309421.1	hypothetical protein	-
CI661_RS29025	29876..30844	+	969	WP_012457123.1	DUF1738 domain-containing protein	-
CI661_RS29030	31028..31558	-	531	WP_012457122.1	phospholipase D family protein	-
CI661_RS29035	31661..32416	-	756	WP_023316386.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36884	36884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 15014.38 Da
• Isoelectric Point: 12.8644

>T127595 WP_012457127.1 NZ_CP041104:27800-28150 [Klebsiella pneumoniae subsp. pneumoniae]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 351 bp

>T127595 NZ_CP041104:27800-28150 [Klebsiella pneumoniae subsp. pneumoniae]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 12835.43 Da
• Isoelectric Point: 12.7956

>AT127595 WP_012457126.1 NZ_CP041104:28153-28452 [Klebsiella pneumoniae subsp. pneumoniae]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 300 bp

>AT127595 NZ_CP041104:28153-28452 [Klebsiella pneumoniae subsp. pneumoniae]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: B2CBE9

Antitoxin

• AlphaFold DB: B2CBE8