Detailed information of TA system TA127563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13573..14230
• Replicon: plasmid pKp202_1
• Accession: NZ_CP041083
• Organism: Klebsiella pneumoniae strain Kp202

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FJV42_RS27045
• Protein ID: WP_000270043.1
• Coordinates: 13880..14230 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FJV42_RS27040
• Protein ID: WP_000124640.1
• Coordinates: 13573..13875 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJV42_RS26990	9198..9626	+	429	WP_000591074.1	hypothetical protein	-
FJV42_RS26995	9683..10042	+	360	WP_000422768.1	hypothetical protein	-
FJV42_RS27000	10042..10488	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
FJV42_RS27005	10485..11003	+	519	WP_000210756.1	nitrite reductase	-
FJV42_RS27010	11003..11233	+	231	WP_000972663.1	hypothetical protein	-
FJV42_RS27015	11220..12077	+	858	WP_001167032.1	hypothetical protein	-
FJV42_RS27025	12308..12835	+	528	WP_001236377.1	thermonuclease family protein	-
FJV42_RS27030	12893..13165	+	273	WP_001043047.1	HU family DNA-binding protein	-
FJV42_RS27035	13253..13546	+	294	WP_001239997.1	chromosome segregation protein ParM	-
FJV42_RS27040	13573..13875	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FJV42_RS27045	13880..14230	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FJV42_RS27050	14393..14941	+	549	WP_001061574.1	transcriptional regulator	-
FJV42_RS27060	15282..15476	+	195	WP_000343597.1	hypothetical protein	-
FJV42_RS27065	15487..15858	+	372	WP_000516916.1	hypothetical protein	-
FJV42_RS27070	15851..16321	+	471	WP_001281821.1	hypothetical protein	-
FJV42_RS27075	16336..16671	-	336	WP_000683476.1	hypothetical protein	-
FJV42_RS27080	16768..17256	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FJV42_RS27085	17259..17756	+	498	WP_000062185.1	hypothetical protein	-
FJV42_RS27095	17971..18675	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
FJV42_RS27100	18982..19161	+	180	Protein_30	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-16 / blaTEM-1A / blaCTX-M-15 / mph(E) / msr(E) / armA / sul1 / blaNDM-1 / aph(3')-VI / qacE / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / dfrA14	-	1..179254	179254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T127563 WP_000270043.1 NZ_CP041083:c14230-13880 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T127563 NZ_CP041083:c14230-13880 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT127563 WP_000124640.1 NZ_CP041083:c13875-13573 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT127563 NZ_CP041083:c13875-13573 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9