Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4556172..4556838 Replicon chromosome
Accession NZ_CP041051
Organism Citrobacter sp. CF971

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag E6P06_RS25640 Protein ID WP_003847996.1
Coordinates 4556172..4556489 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag E6P06_RS25645 Protein ID WP_003837894.1
Coordinates 4556542..4556838 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6P06_RS25625 4551347..4552225 + 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -
E6P06_RS25630 4552330..4554048 - 1719 WP_139935831.1 DUF4153 domain-containing protein -
E6P06_RS25635 4554427..4556049 + 1623 WP_003023529.1 phosphoenolpyruvate carboxykinase (ATP) -
E6P06_RS25640 4556172..4556489 + 318 WP_003847996.1 hypothetical protein Toxin
E6P06_RS25645 4556542..4556838 + 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
E6P06_RS25650 4556895..4558247 - 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
E6P06_RS25655 4558244..4558963 - 720 WP_001157751.1 two-component system response regulator OmpR -
E6P06_RS25660 4559189..4559662 + 474 WP_003023524.1 transcription elongation factor GreB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T127528 WP_003847996.1 NZ_CP041051:4556172-4556489 [Citrobacter sp. CF971]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T127528 NZ_CP041051:4556172-4556489 [Citrobacter sp. CF971]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT127528 WP_003837894.1 NZ_CP041051:4556542-4556838 [Citrobacter sp. CF971]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT127528 NZ_CP041051:4556542-4556838 [Citrobacter sp. CF971]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References