Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2447226..2447788 Replicon chromosome
Accession NZ_CP041051
Organism Citrobacter sp. CF971

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5QCT4
Locus tag E6P06_RS15255 Protein ID WP_003836234.1
Coordinates 2447226..2447504 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B5QC60
Locus tag E6P06_RS15260 Protein ID WP_003836231.1
Coordinates 2447504..2447788 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6P06_RS15235 2442304..2442735 - 432 WP_003020036.1 peroxiredoxin OsmC -
E6P06_RS15240 2442963..2443103 + 141 WP_003020039.1 stationary-phase-induced ribosome-associated protein -
E6P06_RS15245 2443283..2444980 + 1698 WP_003836239.1 malate dehydrogenase -
E6P06_RS15250 2445290..2447005 + 1716 WP_139935416.1 ATP-binding cassette domain-containing protein -
E6P06_RS15255 2447226..2447504 + 279 WP_003836234.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E6P06_RS15260 2447504..2447788 + 285 WP_003836231.1 HigA family addiction module antidote protein Antitoxin
E6P06_RS15265 2447832..2448434 - 603 WP_003843693.1 inorganic diphosphatase -
E6P06_RS15270 2448564..2449220 - 657 WP_003020054.1 formate dehydrogenase-N subunit gamma -
E6P06_RS15275 2449213..2450097 - 885 WP_003833024.1 formate dehydrogenase N subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10637.28 Da        Isoelectric Point: 9.8965

>T127518 WP_003836234.1 NZ_CP041051:2447226-2447504 [Citrobacter sp. CF971]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGDRDGIWAIAVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T127518 NZ_CP041051:2447226-2447504 [Citrobacter sp. CF971]
ATGATCATGAACTTCAGACACAAGGGATTGCGAGATTTGTTTCTTCATGGAAGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGGCTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGATATCAATATGCCCGGTTATAAGCTAC
ATCCTCTGGTAGGTGATCGTGATGGCATATGGGCAATAGCTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGACTACGAGGACTATCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10358.10 Da        Isoelectric Point: 8.8094

>AT127518 WP_003836231.1 NZ_CP041051:2447504-2447788 [Citrobacter sp. CF971]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQTTWSLAEAQ
KSVDISRLRKLAVQ

Download         Length: 285 bp

>AT127518 NZ_CP041051:2447504-2447788 [Citrobacter sp. CF971]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCTTCAACGGCAAGTCGACTATTAACCGGGAAAGCAGCCCTAACGCCTGAGATGGCGA
TTAAACTCTCTGTAGTGATTGGCAGCTCACCCGAAATGTGGTTAAACCTGCAAACTACCTGGAGTCTGGCAGAAGCCCAA
AAAAGCGTGGATATTTCACGCCTGCGTAAGCTGGCAGTACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5QCT4

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5QC60

References