Detailed information of TA system TA127515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 451736..452406
• Replicon: chromosome
• Accession: NZ_CP041051
• Organism: Citrobacter sp. CF971

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: E6P06_RS05675
• Protein ID: WP_046671374.1
• Coordinates: 451736..452038 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E6P06_RS05680
• Protein ID: WP_046671375.1
• Coordinates: 452065..452406 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E6P06_RS05655	447279..448802	-	1524	WP_139935035.1	sugar ABC transporter ATP-binding protein	-
E6P06_RS05665	448917..451181	+	2265	WP_115489424.1	hybrid sensor histidine kinase/response regulator	-
E6P06_RS05670	451251..451580	+	330	WP_003031807.1	hypothetical protein	-
E6P06_RS05675	451736..452038	+	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E6P06_RS05680	452065..452406	+	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
E6P06_RS05685	452459..453217	-	759	WP_103849062.1	phosphonate metabolism protein PhnP	-
E6P06_RS05690	453226..453660	-	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
E6P06_RS05695	453647..454201	-	555	WP_003841102.1	ribose 1,5-bisphosphokinase	-
E6P06_RS05700	454203..455339	-	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
E6P06_RS05705	455336..456016	-	681	WP_003841104.1	phosphonate C-P lyase system protein PhnL	-
E6P06_RS05710	456106..456864	-	759	WP_003031816.1	phosphonate C-P lyase system protein PhnK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T127515 WP_046671374.1 NZ_CP041051:451736-452038 [Citrobacter sp. CF971]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T127515 NZ_CP041051:451736-452038 [Citrobacter sp. CF971]
ATGTCTAAATCATTAAATATCAGGAGCTTTCGGGATACATGGCTTGAAGATTTTTTTGAGCGGGCGACATCGCACAGAAA
AATACCAGCGGATATTCATACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGGTTAACGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTACTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT127515 WP_046671375.1 NZ_CP041051:452065-452406 [Citrobacter sp. CF971]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT127515 NZ_CP041051:452065-452406 [Citrobacter sp. CF971]
ATGAAACAGGCAACCAGAAAACCCACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAGCCGCTCGGACTGAAAAT
TAACGATCTGGCTGAAATACTGCATGTTCATCGCAATACGGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCCAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACTTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGCATTGTTTCGGTTAAAGATTTTATCGCTCAGCGAAATAG
CGAGTCTAAAAAAGTCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6