Detailed information of TA system TA127484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 340300..340823
• Replicon: chromosome
• Accession: NZ_CP041037
• Organism: Staphylococcus aureus strain NP66

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YCD0
• Locus tag: FIV54_RS01650
• Protein ID: WP_000074043.1
• Coordinates: 340557..340823 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YBB2
• Locus tag: FIV54_RS01645
• Protein ID: WP_000587618.1
• Coordinates: 340300..340557 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIV54_RS01620	335380..336573	+	1194	WP_000675406.1	MFS transporter	-
FIV54_RS01625	336797..337546	-	750	WP_000072463.1	hypothetical protein	-
FIV54_RS01630	337741..339318	+	1578	WP_000143494.1	FMN-binding glutamate synthase family protein	-
FIV54_RS01635	339486..339941	-	456	WP_000732091.1	DUF1307 domain-containing protein	-
FIV54_RS01645	340300..340557	+	258	WP_000587618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FIV54_RS01650	340557..340823	+	267	WP_000074043.1	Txe/YoeB family addiction module toxin	Toxin
FIV54_RS01655	341180..342256	+	1077	WP_001076673.1	M42 family metallopeptidase	-
FIV54_RS01660	342504..343166	+	663	WP_000923527.1	ATP-binding cassette domain-containing protein	-
FIV54_RS01665	343159..343935	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
FIV54_RS01670	344331..345518	+	1188	WP_000026189.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10657.45 Da
• Isoelectric Point: 10.3584

>T127484 WP_000074043.1 NZ_CP041037:340557-340823 [Staphylococcus aureus]
MSNYTVKIKNSAKLDLKKIKHPYLKKTFIEIVETLKNNPYKITQSFEKLEPKYLERYSRRINLQHRVVYTIDDRNKVVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T127484 NZ_CP041037:340557-340823 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9762.78 Da
• Isoelectric Point: 4.1958

>AT127484 WP_000587618.1 NZ_CP041037:340300-340557 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETMYLVSTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT127484 NZ_CP041037:340300-340557 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: T1YCD0

Antitoxin

• AlphaFold DB: T1YBB2