Detailed information of TA system TA127476

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 87403..87657
• Replicon: plasmid pLB_CTX-M-15_PT109
• Accession: NZ_CP041032
• Organism: Escherichia coli strain PT109

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FIR93_RS23700
• Protein ID: WP_015387399.1
• Coordinates: 87403..87609 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	87596..87657 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIR93_RS23670	82650..83561	-	912	WP_000440183.1	carbamate kinase	-
FIR93_RS23675	83572..84792	-	1221	WP_000410951.1	arginine deiminase	-
FIR93_RS23680	85685..86167	+	483	Protein_98	VENN motif pre-toxin domain-containing protein	-
FIR93_RS23685	86113..86559	-	447	Protein_99	incFII family plasmid replication initiator RepA	-
FIR93_RS23690	86552..86626	-	75	WP_032336874.1	RepA leader peptide Tap	-
FIR93_RS23695	86862..87119	-	258	WP_000083833.1	replication regulatory protein RepA	-
FIR93_RS23700	87403..87609	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	87596..87657	+	62	NuclAT_0	-	Antitoxin
-	87596..87657	+	62	NuclAT_0	-	Antitoxin
-	87596..87657	+	62	NuclAT_0	-	Antitoxin
-	87596..87657	+	62	NuclAT_0	-	Antitoxin
FIR93_RS23705	87796..87978	-	183	WP_000968309.1	hypothetical protein	-
FIR93_RS23710	88079..88695	+	617	Protein_104	IS1 family transposase	-
FIR93_RS23715	88733..90304	-	1572	WP_023149734.1	IS66 family transposase	-
FIR93_RS23720	90324..90671	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FIR93_RS23725	90671..91348	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
FIR93_RS24385	91403..91492	+	90	Protein_108	IS1 family transposase	-
FIR93_RS23735	91793..92005	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD	senB / iutA / iucD / iucC / iucB / iucA	1..126409	126409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T127476 WP_015387399.1 NZ_CP041032:c87609-87403 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T127476 NZ_CP041032:c87609-87403 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT127476 NZ_CP041032:87596-87657 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin