Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1838755..1839257 Replicon chromosome
Accession NZ_CP041031
Organism Escherichia coli strain PT109

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FIR93_RS08695 Protein ID WP_000767829.1
Coordinates 1839003..1839257 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FIR93_RS08690 Protein ID WP_001259255.1
Coordinates 1838755..1839006 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIR93_RS08665 1833932..1834999 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FIR93_RS08670 1834999..1836069 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
FIR93_RS08675 1836066..1837370 - 1305 WP_053908451.1 histidinol dehydrogenase -
FIR93_RS08680 1837376..1838275 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FIR93_RS08685 1838421..1838471 - 51 WP_001364200.1 his operon leader peptide -
FIR93_RS08690 1838755..1839006 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FIR93_RS08695 1839003..1839257 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FIR93_RS08700 1839340..1840164 + 825 WP_053908453.1 SDR family oxidoreductase -
FIR93_RS08705 1840210..1841139 + 930 WP_053908455.1 LysR family transcriptional regulator -
FIR93_RS08710 1841354..1841416 + 63 WP_010723108.1 membrane protein YoeI -
FIR93_RS08715 1841406..1842764 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FIR93_RS08720 1842943..1844001 + 1059 WP_053908457.1 transport protein YeeE -
FIR93_RS08725 1844015..1844242 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T127464 WP_000767829.1 NZ_CP041031:1839003-1839257 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T127464 NZ_CP041031:1839003-1839257 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT127464 WP_001259255.1 NZ_CP041031:1838755-1839006 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT127464 NZ_CP041031:1838755-1839006 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References