Detailed information of TA system TA127462

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1115409..1116136
• Replicon: chromosome
• Accession: NZ_CP041031
• Organism: Escherichia coli strain PT109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: FIR93_RS05390
• Protein ID: WP_000547564.1
• Coordinates: 1115409..1115720 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FIR93_RS05395
• Protein ID: WP_000126294.1
• Coordinates: 1115717..1116136 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIR93_RS05360	1110551..1112260	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
FIR93_RS05365	1112270..1112812	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
FIR93_RS05370	1112812..1113579	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
FIR93_RS05375	1113576..1113986	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
FIR93_RS05380	1113979..1114449	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
FIR93_RS05385	1114474..1115235	+	762	WP_001026446.1	hypothetical protein	-
FIR93_RS05390	1115409..1115720	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
FIR93_RS05395	1115717..1116136	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
FIR93_RS05400	1116250..1117674	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
FIR93_RS05405	1117683..1119140	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
FIR93_RS05410	1119400..1120410	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
FIR93_RS05415	1120559..1121086	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T127462 WP_000547564.1 NZ_CP041031:1115409-1115720 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T127462 NZ_CP041031:1115409-1115720 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT127462 WP_000126294.1 NZ_CP041031:1115717-1116136 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT127462 NZ_CP041031:1115717-1116136 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5