Detailed information of TA system TA127383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 480771..481366
• Replicon: chromosome
• Accession: NZ_CP041008
• Organism: Pseudomonas aeruginosa strain FDAARGOS_767

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: FIU18_RS02185
• Protein ID: WP_003113526.1
• Coordinates: 480771..481049 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FIU18_RS02190
• Protein ID: WP_003113527.1
• Coordinates: 481061..481366 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU18_RS02165	476197..477435	+	1239	WP_003113524.1	dipeptidase	-
FIU18_RS02170	477497..478144	+	648	WP_003095021.1	carbonate dehydratase	-
FIU18_RS02175	478214..480442	-	2229	WP_003113525.1	TonB-dependent receptor	-
FIU18_RS02185	480771..481049	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU18_RS02190	481061..481366	+	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
FIU18_RS02195	481730..482803	-	1074	WP_003113528.1	serine/threonine protein kinase	-
FIU18_RS02200	482800..483849	-	1050	WP_003113529.1	serine/threonine protein kinase	-
FIU18_RS02205	483852..484958	-	1107	WP_193218117.1	protein phosphatase 2C domain-containing protein	-
FIU18_RS02215	485488..485760	+	273	WP_003115921.1	hypothetical protein	-
FIU18_RS02225	485972..486262	+	291	WP_033992920.1	DUF5447 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T127383 WP_003113526.1 NZ_CP041008:480771-481049 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T127383 NZ_CP041008:480771-481049 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT127383 WP_003113527.1 NZ_CP041008:481061-481366 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT127383 NZ_CP041008:481061-481366 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3