Detailed information of TA system TA127377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4615311..4615913
• Replicon: chromosome
• Accession: NZ_CP041002
• Organism: Escherichia coli strain FDAARGOS_772

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FIU16_RS23775
• Protein ID: WP_000897305.1
• Coordinates: 4615311..4615622 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FIU16_RS23780
• Protein ID: WP_000356397.1
• Coordinates: 4615623..4615913 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU16_RS23750	4611225..4611824	+	600	WP_001295269.1	glucose-1-phosphatase	-
FIU16_RS23755	4611818..4612690	+	873	WP_000920762.1	virulence factor BrkB family protein	-
FIU16_RS23760	4612687..4613124	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
FIU16_RS23765	4613169..4614110	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FIU16_RS23770	4614174..4615082	-	909	WP_001387201.1	alpha/beta hydrolase	-
FIU16_RS23775	4615311..4615622	+	312	WP_000897305.1	hypothetical protein	Toxin
FIU16_RS23780	4615623..4615913	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
FIU16_RS23785	4616499..4616717	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
FIU16_RS23790	4616937..4617179	+	243	WP_001087409.1	hypothetical protein	-
FIU16_RS23795	4617509..4618438	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FIU16_RS23800	4618435..4619070	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FIU16_RS23805	4619067..4619969	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T127377 WP_000897305.1 NZ_CP041002:4615311-4615622 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T127377 NZ_CP041002:4615311-4615622 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT127377 WP_000356397.1 NZ_CP041002:4615623-4615913 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT127377 NZ_CP041002:4615623-4615913 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7