Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2462118..2462620 Replicon chromosome
Accession NZ_CP041002
Organism Escherichia coli strain FDAARGOS_772

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FIU16_RS13215 Protein ID WP_000767829.1
Coordinates 2462118..2462372 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FIU16_RS13220 Protein ID WP_001259255.1
Coordinates 2462369..2462620 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FIU16_RS13185 2457133..2457360 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
FIU16_RS13190 2457374..2458432 - 1059 WP_000492324.1 transport protein YeeE -
FIU16_RS13195 2458611..2459969 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FIU16_RS13200 2459959..2460021 - 63 WP_010723108.1 membrane protein YoeI -
FIU16_RS13205 2460236..2461165 - 930 WP_000803366.1 LysR family transcriptional regulator -
FIU16_RS13210 2461211..2462035 - 825 WP_000754758.1 SDR family oxidoreductase -
FIU16_RS13215 2462118..2462372 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FIU16_RS13220 2462369..2462620 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FIU16_RS13225 2462904..2462954 + 51 WP_001364200.1 his operon leader peptide -
FIU16_RS13230 2463100..2463999 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
FIU16_RS13235 2464005..2465309 + 1305 WP_001386882.1 histidinol dehydrogenase -
FIU16_RS13240 2465306..2466376 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
FIU16_RS13245 2466376..2467443 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T127370 WP_000767829.1 NZ_CP041002:c2462372-2462118 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T127370 NZ_CP041002:c2462372-2462118 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT127370 WP_001259255.1 NZ_CP041002:c2462620-2462369 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT127370 NZ_CP041002:c2462620-2462369 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References