Detailed information of TA system TA127292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3619833..3620527
• Replicon: chromosome
• Accession: NZ_CP040927
• Organism: Escherichia coli strain YY76-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FH040_RS17675
• Protein ID: WP_001263493.1
• Coordinates: 3619833..3620231 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FH040_RS17680
• Protein ID: WP_000554757.1
• Coordinates: 3620234..3620527 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH040_RS17645	3614833..3616077	-	1245	WP_000189541.1	esterase FrsA	-
-	3615493..3615573	-	81	NuclAT_11	-	-
-	3615493..3615573	-	81	NuclAT_11	-	-
-	3615493..3615573	-	81	NuclAT_11	-	-
-	3615493..3615573	-	81	NuclAT_11	-	-
FH040_RS17650	3616169..3616627	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FH040_RS17655	3616888..3618345	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FH040_RS17660	3618402..3618923	-	522	Protein_3428	peptide chain release factor H	-
FH040_RS17665	3618922..3619125	-	204	Protein_3429	RNA ligase RtcB family protein	-
FH040_RS17670	3619371..3619823	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FH040_RS17675	3619833..3620231	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FH040_RS17680	3620234..3620527	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FH040_RS17685	3620579..3621634	-	1056	WP_063501914.1	DNA polymerase IV	-
FH040_RS17690	3621705..3622490	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
FH040_RS17695	3622462..3624174	+	1713	Protein_3435	flagellar biosynthesis protein FlhA	-
FH040_RS17700	3624398..3624895	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T127292 WP_001263493.1 NZ_CP040927:c3620231-3619833 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T127292 NZ_CP040927:c3620231-3619833 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT127292 WP_000554757.1 NZ_CP040927:c3620527-3620234 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT127292 NZ_CP040927:c3620527-3620234 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6