Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1737459..1737961 Replicon chromosome
Accession NZ_CP040927
Organism Escherichia coli strain YY76-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FH040_RS08215 Protein ID WP_000767829.1
Coordinates 1737707..1737961 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FH040_RS08210 Protein ID WP_001259255.1
Coordinates 1737459..1737710 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FH040_RS08185 1732637..1733704 - 1068 WP_148245887.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FH040_RS08190 1733704..1734774 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
FH040_RS08195 1734771..1736075 - 1305 WP_137456045.1 histidinol dehydrogenase -
FH040_RS08200 1736081..1736980 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FH040_RS08205 1737126..1737176 - 51 WP_001364200.1 his operon leader peptide -
FH040_RS08210 1737459..1737710 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FH040_RS08215 1737707..1737961 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FH040_RS08220 1738044..1738868 + 825 WP_000754737.1 SDR family oxidoreductase -
FH040_RS08225 1738914..1739843 + 930 WP_000803351.1 LysR family transcriptional regulator -
FH040_RS08230 1740058..1740120 + 63 WP_010723108.1 membrane protein YoeI -
FH040_RS08235 1740110..1741468 + 1359 WP_024198437.1 putrescine/proton symporter PlaP -
FH040_RS08240 1741647..1742705 + 1059 WP_074511709.1 transport protein YeeE -
FH040_RS08245 1742719..1742946 + 228 WP_096310218.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T127280 WP_000767829.1 NZ_CP040927:1737707-1737961 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T127280 NZ_CP040927:1737707-1737961 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT127280 WP_001259255.1 NZ_CP040927:1737459-1737710 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT127280 NZ_CP040927:1737459-1737710 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References