Detailed information of TA system TA127274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 650387..651114
• Replicon: chromosome
• Accession: NZ_CP040927
• Organism: Escherichia coli strain YY76-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5P0J3S6
• Locus tag: FH040_RS03160
• Protein ID: WP_063501682.1
• Coordinates: 650387..650701 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FH040_RS03165
• Protein ID: WP_000560266.1
• Coordinates: 650698..651114 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH040_RS03140	646545..647531	-	987	WP_000617698.1	Gfo/Idh/MocA family oxidoreductase	-
FH040_RS03145	647610..648302	-	693	WP_063501683.1	vancomycin high temperature exclusion protein	-
FH040_RS03150	648379..648882	-	504	WP_001333820.1	M48 family metallopeptidase	-
FH040_RS03155	648967..650103	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
FH040_RS03160	650387..650701	+	315	WP_063501682.1	type II toxin-antitoxin system toxin HigB	Toxin
FH040_RS03165	650698..651114	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
FH040_RS03170	651159..653177	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
FH040_RS03175	653603..655954	-	2352	WP_000695487.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12202.24 Da
• Isoelectric Point: 10.1604

>T127274 WP_063501682.1 NZ_CP040927:650387-650701 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALRNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T127274 NZ_CP040927:650387-650701 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGAG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT127274 WP_000560266.1 NZ_CP040927:650698-651114 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT127274 NZ_CP040927:650698-651114 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A5P0J3S6