Detailed information of TA system TA127270

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19728..19992
• Replicon: plasmid p853EC4
• Accession: NZ_CP040923
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: FH485_RS27635
• Protein ID: WP_001387489.1
• Coordinates: 19840..19992 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	19728..19788 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS27600	15854..16117	+	264	WP_032180564.1	ash family protein	-
FH485_RS27605	16035..16322	+	288	WP_000074855.1	conjugal transfer protein TraA	-
FH485_RS27615	16764..17069	+	306	Protein_23	transcription termination factor NusG	-
FH485_RS27620	17083..17779	-	697	Protein_24	IS1 family transposase	-
FH485_RS27625	17835..18992	-	1158	Protein_25	IS1380-like element ISEc9 family transposase	-
FH485_RS27630	19178..19525	-	348	Protein_26	protein finQ	-
-	19728..19788	-	61	NuclAT_0	-	Antitoxin
-	19728..19788	-	61	NuclAT_0	-	Antitoxin
-	19728..19788	-	61	NuclAT_0	-	Antitoxin
-	19728..19788	-	61	NuclAT_0	-	Antitoxin
FH485_RS27635	19840..19992	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
FH485_RS27640	20064..20315	-	252	WP_001291964.1	hypothetical protein	-
FH485_RS27645	20615..20911	+	297	WP_011264046.1	hypothetical protein	-
FH485_RS28045	20976..21152	-	177	WP_001054897.1	hypothetical protein	-
FH485_RS27650	21544..21753	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
FH485_RS27655	21978..22675	+	698	Protein_32	IS1 family transposase	-
FH485_RS27660	23128..24273	+	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
FH485_RS27665	24367..24900	+	534	WP_001221666.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..45224	45224
	-	inside	IScluster/Tn	blaCMY-42	-	17504..24273	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T127270 WP_001387489.1 NZ_CP040923:19840-19992 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T127270 NZ_CP040923:19840-19992 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT127270 NZ_CP040923:c19788-19728 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin