Detailed information of TA system TA127263

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34854..35093
• Replicon: plasmid p853EC3
• Accession: NZ_CP040922
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: FH485_RS26780
• Protein ID: WP_023144756.1
• Coordinates: 34959..35093 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	34854..34914 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS26755	32384..33130	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
FH485_RS26760	33185..33745	+	561	WP_000139341.1	fertility inhibition protein FinO	-
FH485_RS26765	33876..34088	+	213	WP_013023861.1	hypothetical protein	-
FH485_RS26775	34601..34887	+	287	Protein_38	DUF2726 domain-containing protein	-
-	34854..34914	-	61	NuclAT_2	-	Antitoxin
-	34854..34914	-	61	NuclAT_2	-	Antitoxin
-	34854..34914	-	61	NuclAT_2	-	Antitoxin
-	34854..34914	-	61	NuclAT_2	-	Antitoxin
FH485_RS26780	34959..35093	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
FH485_RS26785	35390..35500	+	111	Protein_40	replication protein	-
FH485_RS26790	35569..36348	-	780	WP_001323403.1	IS21-like element IS100kyp family helper ATPase IstB	-
FH485_RS26795	36348..37370	-	1023	WP_000255944.1	IS21-like element IS100 family transposase	-
FH485_RS26800	37451..37603	+	153	WP_072258247.1	replication protein A	-
FH485_RS26805	37840..37914	+	75	WP_031943482.1	RepA leader peptide Tap	-
FH485_RS26810	37907..38764	+	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
FH485_RS28025	39466..39606	+	141	WP_001333237.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / mph(A) / erm(B) / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..143714	143714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T127263 WP_023144756.1 NZ_CP040922:34959-35093 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T127263 NZ_CP040922:34959-35093 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT127263 NZ_CP040922:c34914-34854 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin