Detailed information of TA system TA127260

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 10458..10727
• Replicon: plasmid p853EC3
• Accession: NZ_CP040922
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FH485_RS26630
• Protein ID: WP_001312861.1
• Coordinates: 10569..10727 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	10458..10523 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS26605	5679..7637	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
FH485_RS26610	7692..8126	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
FH485_RS26615	8123..8842	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	8854..8956	+	103	NuclAT_1	-	-
-	8854..8956	+	103	NuclAT_1	-	-
-	8854..8956	+	103	NuclAT_1	-	-
-	8854..8956	+	103	NuclAT_1	-	-
FH485_RS26620	9002..10371	+	1370	WP_140028060.1	IS3-like element IS150 family transposase	-
-	10398..10525	+	128	NuclAT_0	-	-
-	10398..10525	+	128	NuclAT_0	-	-
-	10398..10525	+	128	NuclAT_0	-	-
-	10398..10525	+	128	NuclAT_0	-	-
-	10458..10523	+	66	-	-	Antitoxin
FH485_RS26630	10569..10727	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FH485_RS26650	11504..11623	+	120	WP_032165723.1	hypothetical protein	-
FH485_RS26655	11648..11935	+	288	WP_000107535.1	hypothetical protein	-
FH485_RS26660	12054..12875	+	822	WP_001234469.1	DUF945 domain-containing protein	-
FH485_RS26665	13172..13774	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
FH485_RS26670	14105..14488	+	384	WP_001151566.1	relaxosome protein TraM	-
FH485_RS26675	14622..15299	+	678	WP_001348626.1	PAS domain-containing protein	-
FH485_RS26680	15387..15614	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / mph(A) / erm(B) / tet(B) / sitABCD	iutA / iucD / iucC / iucB / iucA	1..143714	143714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T127260 WP_001312861.1 NZ_CP040922:10569-10727 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T127260 NZ_CP040922:10569-10727 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT127260 NZ_CP040922:10458-10523 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin