Detailed information of TA system TA127258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 34059..34705
• Replicon: plasmid p853EC2
• Accession: NZ_CP040921
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FH485_RS26445
• Protein ID: WP_000269912.1
• Coordinates: 34358..34705 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1V3UZT0
• Locus tag: FH485_RS26440
• Protein ID: WP_001259436.1
• Coordinates: 34059..34358 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS26410	29976..30167	+	192	WP_000183351.1	hypothetical protein	-
FH485_RS26415	30164..31306	+	1143	WP_032154032.1	ORF6N domain-containing protein	-
FH485_RS26420	31341..31976	-	636	WP_032154031.1	hypothetical protein	-
FH485_RS26425	32633..32884	-	252	WP_039052397.1	helix-turn-helix domain-containing protein	-
FH485_RS26430	32986..33411	+	426	WP_016240784.1	hypothetical protein	-
FH485_RS26435	33515..33895	-	381	WP_001523084.1	hypothetical protein	-
FH485_RS26440	34059..34358	-	300	WP_001259436.1	XRE family transcriptional regulator	Antitoxin
FH485_RS26445	34358..34705	-	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FH485_RS26450	34950..35213	-	264	WP_000424604.1	hypothetical protein	-
FH485_RS26455	35237..35524	-	288	WP_000356589.1	hypothetical protein	-
FH485_RS26460	36168..37004	-	837	WP_024171747.1	replication initiator protein RepA	-
FH485_RS26465	37630..38175	+	546	WP_001704996.1	transferase	-
FH485_RS26470	38139..38756	-	618	WP_001523080.1	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49465	49465

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T127258 WP_000269912.1 NZ_CP040921:c34705-34358 [Escherichia coli]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T127258 NZ_CP040921:c34705-34358 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATCAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAACGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11074.92 Da
• Isoelectric Point: 5.2090

>AT127258 WP_001259436.1 NZ_CP040921:c34358-34059 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT127258 NZ_CP040921:c34358-34059 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCTGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1V3UZT0