Detailed information of TA system TA127247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3842641..3843335
• Replicon: chromosome
• Accession: NZ_CP040919
• Organism: Escherichia coli strain FC853_EC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: FH485_RS18980
• Protein ID: WP_001263491.1
• Coordinates: 3842641..3843039 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: FH485_RS18985
• Protein ID: WP_000554755.1
• Coordinates: 3843042..3843335 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FH485_RS18950	3837903..3839360	+	1458	WP_022645227.1	cytosol nonspecific dipeptidase	-
FH485_RS18955	3839369..3839650	+	282	WP_022645226.1	hypothetical protein	-
FH485_RS18960	3839667..3840176	-	510	WP_001361775.1	hydrolase	-
FH485_RS18965	3840238..3840852	-	615	WP_022645225.1	peptide chain release factor H	-
FH485_RS18970	3840849..3841988	-	1140	WP_022645224.1	RNA ligase RtcB family protein	-
FH485_RS18975	3842179..3842631	-	453	WP_023909048.1	GNAT family N-acetyltransferase	-
FH485_RS18980	3842641..3843039	-	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FH485_RS18985	3843042..3843335	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FH485_RS18990	3843387..3844442	-	1056	WP_022645222.1	DNA polymerase IV	-
FH485_RS18995	3844513..3845298	-	786	WP_072224360.1	putative lateral flagellar export/assembly protein LafU	-
FH485_RS19000	3845243..3846981	+	1739	Protein_3647	flagellar biosynthesis protein FlhA	-
FH485_RS19005	3847079..3847852	-	774	WP_022645219.1	C40 family peptidase	-
FH485_RS19010	3848038..3848298	+	261	WP_000729708.1	type II toxin-antitoxin system antitoxin DinJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3815106..3848510	33404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T127247 WP_001263491.1 NZ_CP040919:c3843039-3842641 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T127247 NZ_CP040919:c3843039-3842641 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT127247 WP_000554755.1 NZ_CP040919:c3843335-3843042 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT127247 NZ_CP040919:c3843335-3843042 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5