Detailed information of TA system TA127214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 17998..18460
• Replicon: plasmid pAB17H194-2
• Accession: NZ_CP040913
• Organism: Acinetobacter pittii strain AB17H194

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3R9PS35
• Locus tag: E8E07_RS19365
• Protein ID: WP_000033474.1
• Coordinates: 18272..18460 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E8E07_RS19360
• Protein ID: WP_001258992.1
• Coordinates: 17998..18285 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8E07_RS19325	13375..13830	+	456	WP_005071565.1	hypothetical protein	-
E8E07_RS19330	13891..14358	+	468	WP_005071563.1	hypothetical protein	-
E8E07_RS19335	14327..14968	+	642	WP_005071561.1	hypothetical protein	-
E8E07_RS19340	15028..15569	+	542	Protein_14	DUF2280 domain-containing protein	-
E8E07_RS19345	15553..16869	+	1317	WP_005071556.1	hypothetical protein	-
E8E07_RS19350	16909..17466	+	558	Protein_16	hypothetical protein	-
E8E07_RS19355	17490..17714	+	225	WP_005071551.1	hypothetical protein	-
E8E07_RS19360	17998..18285	-	288	WP_001258992.1	helix-turn-helix transcriptional regulator	Antitoxin
E8E07_RS19365	18272..18460	-	189	WP_000033474.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E8E07_RS19370	18737..19057	-	321	WP_002123990.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E8E07_RS19375	19242..19630	+	389	Protein_21	AAA family ATPase	-
E8E07_RS19380	19558..20103	+	546	Protein_22	IS5 family transposase	-
E8E07_RS19390	20861..21088	+	228	WP_005071544.1	hypothetical protein	-
E8E07_RS19395	21198..21407	-	210	WP_000069471.1	hypothetical protein	-
E8E07_RS19400	21400..21702	-	303	WP_001140619.1	XRE family transcriptional regulator	-
E8E07_RS19405	21695..22051	-	357	WP_000269903.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E8E07_RS19410	22576..23376	-	801	WP_017398828.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..76962	76962
	-	flank	IS/Tn	-	-	19858..20103	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-55)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-90)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7137.56 Da
• Isoelectric Point: 10.9048

>T127214 WP_000033474.1 NZ_CP040913:c18460-18272 [Acinetobacter pittii]
MSKGLFELRLKSQEGIARVMYCTLVGKRIVMLHSFVKKTQKTPKQDLNLALDRMKEVKNANT

Nucleotide

• Length: 189 bp

>T127214 NZ_CP040913:c18460-18272 [Acinetobacter pittii]
ATGAGTAAAGGACTATTTGAGTTGCGCTTAAAATCTCAAGAAGGTATTGCTCGTGTTATGTATTGCACACTAGTCGGTAA
ACGCATTGTAATGCTCCATAGTTTTGTTAAGAAAACCCAAAAAACACCTAAGCAAGATCTAAACCTAGCACTTGACCGCA
TGAAAGAGGTGAAAAATGCGAACACATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10563.32 Da
• Isoelectric Point: 9.7308

>AT127214 WP_001258992.1 NZ_CP040913:c18285-17998 [Acinetobacter pittii]
MRTHEEMKALALSRSAVRTEYERIEREEMPLLDMVLTALREAGLSQAQIAERMGTNVPAVSRLEKALITGKPSPSIATLQ
KYAAAIGKHVEVRFV

Nucleotide

• Length: 288 bp

>AT127214 NZ_CP040913:c18285-17998 [Acinetobacter pittii]
ATGCGAACACATGAAGAAATGAAAGCACTTGCATTATCTCGTTCAGCAGTGCGTACCGAATACGAACGAATTGAACGTGA
AGAAATGCCCTTGCTCGATATGGTACTTACTGCACTTCGAGAGGCAGGTCTATCCCAAGCGCAAATCGCGGAACGCATGG
GAACAAATGTACCTGCCGTTTCACGGCTAGAAAAAGCATTGATCACAGGCAAACCATCACCATCTATTGCCACATTGCAA
AAATACGCTGCGGCAATTGGTAAACATGTTGAAGTGCGTTTTGTCTAA

Structures

Toxin

• AlphaFold DB: A0A3R9PS35