Detailed information of TA system TA127213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 70460..71153
• Replicon: plasmid pAB17H194-1
• Accession: NZ_CP040912
• Organism: Acinetobacter pittii strain AB17H194

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R9B971
• Locus tag: E8E07_RS19155
• Protein ID: WP_016165531.1
• Coordinates: 70460..70750 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6N0LK42
• Locus tag: E8E07_RS19160
• Protein ID: WP_035268402.1
• Coordinates: 70752..71153 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E8E07_RS19115	65516..65737	+	222	WP_004658364.1	helix-turn-helix transcriptional regulator	-
E8E07_RS19120	65989..66642	+	654	Protein_67	IS5 family transposase	-
E8E07_RS19125	66888..67295	-	408	WP_001219642.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
E8E07_RS19130	67391..68287	+	897	WP_150378257.1	cation transporter	-
E8E07_RS19135	68291..68803	+	513	WP_150378258.1	signal peptidase II	-
E8E07_RS19140	68853..69338	-	486	Protein_71	IS3 family transposase	-
E8E07_RS19145	69444..69989	+	546	WP_127800924.1	DNA-binding protein	-
E8E07_RS19150	70040..70294	-	255	Protein_73	transposase	-
E8E07_RS19155	70460..70750	+	291	WP_016165531.1	type II toxin-antitoxin system HigB family toxin	Toxin
E8E07_RS19160	70752..71153	+	402	WP_035268402.1	helix-turn-helix domain-containing protein	Antitoxin
E8E07_RS19175	72656..73828	-	1173	WP_150378260.1	replication initiation protein	-
E8E07_RS19180	74685..75464	+	780	WP_150378261.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / dfrA1 / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / mph(E) / msr(E) / blaTEM-2 / aac(3)-IIa / tet(X5)	-	1..88002	88002
	-	inside	IScluster/Tn	tet(X5)	-	68853..85964	17111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (19-90)

Antitoxin

• Antitoxin HigA (11-133)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11257.12 Da
• Isoelectric Point: 10.1259

>T127213 WP_016165531.1 NZ_CP040912:70460-70750 [Acinetobacter pittii]
MHVITHARIIEAMHKWPQAETALDGWYRTIKANDPKDFAEMKQLFPAVDKVGKFHVFDIGGNKIRLIAVVMYQAKRVYIR
HVLSHKEYDKGHWKEG

Nucleotide

• Length: 291 bp

>T127213 NZ_CP040912:70460-70750 [Acinetobacter pittii]
ATGCATGTGATCACCCACGCTAGAATTATAGAAGCAATGCACAAGTGGCCACAGGCTGAAACGGCATTAGATGGTTGGTA
TCGAACCATAAAGGCAAACGATCCGAAAGATTTTGCTGAGATGAAACAGCTCTTTCCAGCTGTCGATAAAGTTGGAAAGT
TCCATGTATTCGATATTGGCGGGAACAAGATCCGACTTATTGCCGTCGTGATGTATCAAGCAAAACGTGTGTATATTCGT
CATGTCTTGTCCCATAAAGAATATGACAAAGGTCATTGGAAGGAAGGTTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14823.65 Da
• Isoelectric Point: 4.3676

>AT127213 WP_035268402.1 NZ_CP040912:70752-71153 [Acinetobacter pittii]
MNAMIKQAVDHWHYVAPLLSKPENEDDFHTLVEALDELLDTVGDDESNPLMGLIHQLGDLVSDYENEHLPMPKGDGRAAL
AFLMEQHGLGQSDLAEVATQSVISEILSGKRQLNIRHIKALSERFNVSADTFF

Nucleotide

• Length: 402 bp

>AT127213 NZ_CP040912:70752-71153 [Acinetobacter pittii]
ATGAACGCGATGATTAAACAGGCCGTAGACCACTGGCACTACGTTGCACCATTGCTCAGCAAGCCAGAAAATGAAGACGA
CTTTCATACACTTGTTGAAGCTTTAGATGAACTTTTAGATACCGTTGGTGATGACGAATCTAATCCTTTAATGGGGCTTA
TTCATCAGCTTGGTGATTTGGTTTCTGATTATGAAAATGAGCATCTTCCTATGCCTAAAGGGGATGGTCGTGCTGCTTTG
GCTTTTTTGATGGAGCAACATGGTTTAGGTCAGTCTGACTTAGCAGAAGTTGCAACCCAATCTGTGATTTCTGAGATTTT
AAGTGGTAAACGCCAACTGAATATTCGTCATATTAAGGCATTGTCTGAGCGGTTTAATGTATCGGCAGATACGTTTTTCT
AA

Structures

Toxin

• AlphaFold DB: R9B971

Antitoxin

• AlphaFold DB: A0A6N0LK42