Detailed information of TA system TA127162

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89623..89877
• Replicon: plasmid pK71-77-2
• Accession: NZ_CP040885
• Organism: Escherichia coli strain K71-77

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: FHY70_RS01520
• Protein ID: WP_015387399.1
• Coordinates: 89671..89877 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89623..89684 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY70_RS01475	85275..85487	+	213	WP_005012601.1	hypothetical protein	-
FHY70_RS25810	85788..85877	-	90	Protein_98	IS1 family transposase	-
FHY70_RS01490	85932..86609	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
FHY70_RS01495	86609..86956	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
FHY70_RS01500	86976..88547	+	1572	WP_023149734.1	IS66 family transposase	-
FHY70_RS01505	88585..89201	-	617	Protein_102	IS1 family transposase	-
FHY70_RS01510	89302..89484	+	183	WP_000968309.1	hypothetical protein	-
-	89623..89684	-	62	NuclAT_1	-	Antitoxin
-	89623..89684	-	62	NuclAT_1	-	Antitoxin
-	89623..89684	-	62	NuclAT_1	-	Antitoxin
-	89623..89684	-	62	NuclAT_1	-	Antitoxin
FHY70_RS01520	89671..89877	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
FHY70_RS01525	90161..90418	+	258	WP_000083833.1	replication regulatory protein RepA	-
FHY70_RS01535	90654..90728	+	75	WP_032336874.1	RepA leader peptide Tap	-
FHY70_RS01540	90721..91167	+	447	Protein_107	incFII family plasmid replication initiator RepA	-
FHY70_RS01545	91113..91595	-	483	Protein_108	VENN motif pre-toxin domain-containing protein	-
FHY70_RS01550	92488..93708	+	1221	WP_000410951.1	arginine deiminase	-
FHY70_RS01555	93719..94630	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(6')-Ib-cr / blaOXA-1	iucA / iucB / iucC / iucD / iutA	1..117590	117590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T127162 WP_015387399.1 NZ_CP040885:89671-89877 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T127162 NZ_CP040885:89671-89877 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT127162 NZ_CP040885:c89684-89623 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin