Detailed information of TA system TA127158

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49531..49801
• Replicon: plasmid pK71-77-2
• Accession: NZ_CP040885
• Organism: Escherichia coli strain K71-77

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: FHY70_RS01270
• Protein ID: WP_001312861.1
• Coordinates: 49643..49801 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49531..49594 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY70_RS01220	45654..46219	+	566	Protein_49	class I SAM-dependent methyltransferase	-
FHY70_RS01230	46370..46621	+	252	WP_071579736.1	hypothetical protein	-
FHY70_RS01240	46861..47067	+	207	WP_000275856.1	hypothetical protein	-
FHY70_RS01245	47093..47632	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
FHY70_RS01250	47700..47933	+	234	WP_000005990.1	DUF905 family protein	-
FHY70_RS01255	47961..48158	+	198	Protein_54	hypothetical protein	-
FHY70_RS01260	48213..48647	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
FHY70_RS01265	48644..49363	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
FHY70_RS25805	49375..49563	-	189	WP_001299721.1	hypothetical protein	-
-	49375..49599	+	225	NuclAT_0	-	-
-	49375..49599	+	225	NuclAT_0	-	-
-	49375..49599	+	225	NuclAT_0	-	-
-	49375..49599	+	225	NuclAT_0	-	-
-	49531..49594	-	64	-	-	Antitoxin
FHY70_RS01270	49643..49801	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
FHY70_RS01285	50722..51009	+	288	WP_000107535.1	hypothetical protein	-
FHY70_RS01290	51127..51948	+	822	WP_001234426.1	DUF945 domain-containing protein	-
FHY70_RS01295	52245..52847	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
FHY70_RS01300	53168..53551	+	384	WP_001151524.1	relaxosome protein TraM	-
FHY70_RS01305	53738..54427	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aac(6')-Ib-cr / blaOXA-1	iucA / iucB / iucC / iucD / iutA	1..117590	117590

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T127158 WP_001312861.1 NZ_CP040885:49643-49801 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T127158 NZ_CP040885:49643-49801 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT127158 NZ_CP040885:c49594-49531 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin