Detailed information of TA system TA127155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 131063..131720
• Replicon: plasmid pK71-77-1-NDM
• Accession: NZ_CP040884
• Organism: Escherichia coli strain K71-77

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: FHY70_RS00810
• Protein ID: WP_000270043.1
• Coordinates: 131370..131720 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FHY70_RS00805
• Protein ID: WP_000124640.1
• Coordinates: 131063..131365 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FHY70_RS00755	126697..127125	+	429	WP_000591076.1	hypothetical protein	-
FHY70_RS00760	127183..127542	+	360	WP_000422769.1	hypothetical protein	-
FHY70_RS00765	127542..127988	+	447	WP_000919343.1	hypothetical protein	-
FHY70_RS00770	127985..128503	+	519	WP_000210757.1	nitrite reductase	-
FHY70_RS00775	128503..128733	+	231	WP_000972665.1	hypothetical protein	-
FHY70_RS00780	128720..129577	+	858	WP_001167036.1	hypothetical protein	-
FHY70_RS25795	129489..129794	+	306	WP_171820680.1	hypothetical protein	-
FHY70_RS00790	129797..130324	+	528	WP_004201083.1	thermonuclease family protein	-
FHY70_RS00795	130382..130654	+	273	WP_001043046.1	HU family DNA-binding protein	-
FHY70_RS00800	130743..131036	+	294	WP_001239998.1	hypothetical protein	-
FHY70_RS00805	131063..131365	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
FHY70_RS00810	131370..131720	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FHY70_RS00815	131883..132431	+	549	WP_001061573.1	hypothetical protein	-
FHY70_RS00825	132772..132966	+	195	WP_000343597.1	hypothetical protein	-
FHY70_RS00830	132977..133348	+	372	WP_000516918.1	hypothetical protein	-
FHY70_RS00835	133341..133811	+	471	WP_001281821.1	hypothetical protein	-
FHY70_RS00840	133826..134161	-	336	WP_000683477.1	hypothetical protein	-
FHY70_RS00845	134258..134746	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
FHY70_RS00850	134749..135246	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-6 / aac(6')-Ib / qacE / sul1 / rmtC / aac(3)-IId / blaNDM-1	htpB	1..145272	145272

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T127155 WP_000270043.1 NZ_CP040884:c131720-131370 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T127155 NZ_CP040884:c131720-131370 [Escherichia coli]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT127155 WP_000124640.1 NZ_CP040884:c131365-131063 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT127155 NZ_CP040884:c131365-131063 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9