Detailed information of TA system TA127117

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3272180..3272826
• Replicon: chromosome
• Accession: NZ_CP040861
• Organism: Klebsiella pneumoniae strain Xen39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FEE38_RS16580
• Protein ID: WP_117096712.1
• Coordinates: 3272180..3272527 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8UB68
• Locus tag: FEE38_RS16585
• Protein ID: WP_002920557.1
• Coordinates: 3272527..3272826 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FEE38_RS16570	3268106..3269539	+	1434	WP_002920564.1	glycogen synthase GlgA	-
FEE38_RS16575	3269557..3272004	+	2448	WP_002920561.1	glycogen phosphorylase	-
FEE38_RS16580	3272180..3272527	+	348	WP_117096712.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FEE38_RS16585	3272527..3272826	+	300	WP_002920557.1	helix-turn-helix transcriptional regulator	Antitoxin
FEE38_RS16590	3272889..3274397	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
FEE38_RS16595	3274602..3274931	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
FEE38_RS16600	3274982..3275812	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
FEE38_RS16605	3275862..3276620	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13474.51 Da
• Isoelectric Point: 6.2300

>T127117 WP_117096712.1 NZ_CP040861:3272180-3272527 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T127117 NZ_CP040861:3272180-3272527 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.58 Da
• Isoelectric Point: 10.1464

>AT127117 WP_002920557.1 NZ_CP040861:3272527-3272826 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT127117 NZ_CP040861:3272527-3272826 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1CBF8