Detailed information of TA system TA127021

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70854..71280
• Replicon: plasmid pEF02
• Accession: NZ_CP040807
• Organism: Escherichia fergusonii strain EFCF056

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: D8Z79_RS23930
• Protein ID: WP_001312861.1
• Coordinates: 70854..71012 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	71056..71280 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z79_RS23885	65909..66136	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
D8Z79_RS23890	66230..66916	-	687	WP_015059009.1	PAS domain-containing protein	-
D8Z79_RS23895	67107..67490	-	384	WP_000124981.1	relaxosome protein TraM	-
D8Z79_RS23900	67767..68414	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
D8Z79_RS23905	68711..69532	-	822	WP_001234445.1	DUF945 domain-containing protein	-
D8Z79_RS23910	69643..69939	-	297	WP_001272251.1	hypothetical protein	-
D8Z79_RS26170	70239..70616	+	378	Protein_85	hypothetical protein	-
D8Z79_RS23930	70854..71012	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	71056..71280	-	225	NuclAT_0	-	Antitoxin
-	71056..71280	-	225	NuclAT_0	-	Antitoxin
-	71056..71280	-	225	NuclAT_0	-	Antitoxin
-	71056..71280	-	225	NuclAT_0	-	Antitoxin
D8Z79_RS26175	71092..71271	+	180	WP_001309233.1	hypothetical protein	-
D8Z79_RS23935	71292..72011	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
D8Z79_RS23940	72008..72442	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
D8Z79_RS23945	72511..74534	-	2024	Protein_90	ParB/RepB/Spo0J family partition protein	-
D8Z79_RS23950	74595..74828	-	234	WP_000006003.1	DUF905 family protein	-
D8Z79_RS23955	74886..75413	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
D8Z79_RS26180	75715..76170	+	456	Protein_93	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..90871	90871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T127021 WP_001312861.1 NZ_CP040807:c71012-70854 [Escherichia fergusonii]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T127021 NZ_CP040807:c71012-70854 [Escherichia fergusonii]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT127021 NZ_CP040807:c71280-71056 [Escherichia fergusonii]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin