Detailed information of TA system TA127017

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31393..31646
• Replicon: plasmid pEF02
• Accession: NZ_CP040807
• Organism: Escherichia fergusonii strain EFCF056

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: D8Z79_RS23685
• Protein ID: WP_001312851.1
• Coordinates: 31393..31542 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31587..31646 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z79_RS23640	26752..27167	-	416	Protein_33	IS1 family transposase	-
D8Z79_RS23645	27416..27817	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
D8Z79_RS23650	27750..28007	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
D8Z79_RS23655	28100..28753	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
D8Z79_RS23665	29692..30549	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
D8Z79_RS23670	30542..30616	-	75	WP_001375168.1	RepA leader peptide Tap	-
D8Z79_RS23680	30861..31109	-	249	WP_000083837.1	replication regulatory protein RepA	-
D8Z79_RS23685	31393..31542	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	31587..31646	+	60	NuclAT_1	-	Antitoxin
-	31587..31646	+	60	NuclAT_1	-	Antitoxin
-	31587..31646	+	60	NuclAT_1	-	Antitoxin
-	31587..31646	+	60	NuclAT_1	-	Antitoxin
D8Z79_RS23695	31847..32077	-	231	WP_001736714.1	hypothetical protein	-
D8Z79_RS23700	32241..32840	-	600	WP_032083981.1	hypothetical protein	-
D8Z79_RS23705	33226..33426	-	201	WP_015059022.1	hypothetical protein	-
D8Z79_RS23710	33558..34118	-	561	WP_000139328.1	fertility inhibition protein FinO	-
D8Z79_RS23715	34173..34919	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / fosA3 / rmtB / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	-	1..90871	90871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T127017 WP_001312851.1 NZ_CP040807:c31542-31393 [Escherichia fergusonii]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T127017 NZ_CP040807:c31542-31393 [Escherichia fergusonii]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT127017 NZ_CP040807:31587-31646 [Escherichia fergusonii]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin